DISCOVER

Kaer Imaging System

OPEN FLUORESCENCE IMAGING SYSTEM

The Kaer Imaging System is a stand alone Open Fluorescence In Vivo Imaging System. It is designed for the detection of near infrared fluorescent molecules in animals in real time. Its optical head can be hand held or fixed on a stand, depending on the setup of the study. The system can be used on both small and large animals and is adapted to intraoperative imaging.

The system is designed for preclinical research only and should not be used for clinical use

Imaging of subcutaneous tumors in a mouse model of pancreatic cancer with a bimodal PET-fluorescent tracer. Courtesy of Aurélie Prignon, Plateforme LIMP, UMS 28, Faculté de médecine Sorbonne Université, Paris, France, and Emma Renard, Institut de Chimie Moléculaire de l’Université de Bourgogne, UMR CNRS 6302, Université Bourgogne Franche-Comté, Dijon, France.

Open fluorescence imaging experiments with Dr. Aurélie Prignon at the LIMP imaging platform in Paris, developing new imaging agents for fluorescence guided surgery with the Kaer Imaging System

​Specifications

KIS 700: excitation 640 nm - collection: < 665 nm (high pass)

KIS 800: excitation 785 nm - collection: < 800 nm (high pass)

Excitation type: laser

Field of view: 6.4 x 6.4 cm2

Image size (pixels): 1024 x 1024

Number of bits: 16

Image format: TIFF

Export format: AVI

 

Flexibility

Open design

Optical head - hand held or fixed on a stand

Dynamic imaging

Portable instrumentation

Can be controlled by a laptop

Sensitivity

Laser excitation

High power due to limited working distance

Dedicated filters to maximize sensitivity for a given wavelength (no filter wheel)

Near infrared fluorescence for better tissue propagation

Background recording and subtraction from fluorescence signal in real time

Pseudo colors and overlay in real time for easier image interpretation

 

Quantification

Linear detection for quantification of the signal

High dynamic range of the detector, for quantification of weak and strong signals

On the fly quantification of ROI: histograms and signal kinetics

Tif image format recording for post acquisition quantification

 

User friendliness

Very easy to install and use

Only one parameter to adjust to optimize image quality and sensitivity

No proprietary image format, post acquisition analysis is possible with any scientific image processing software

Bibliography

Here is the list of publications related to the KIS for Near Infrared fluorescence. The complete list of publications, including NIR-II fluorescence related articles, can be found here.

Privat M, Bellaye PS, Lescure R, Massot A, Baffroy O, Moreau M, Racoeur C, Marcion G, Denat F, Bettaieb A, Collin B, Bodio E, Paul C, Goze C. Development of an Easily Bioconjugatable Water-Soluble Single-Photon Emission-Computed Tomography/Optical Imaging Bimodal Imaging Probe Based on the aza-BODIPY Fluorophore. J Med Chem. 2021 Aug 2. doi: 10.1021/acs.jmedchem.1c00450. Epub ahead of print. PMID: 34338511.

https://doi.org/10.1021/acs.jmedchem.1c00450

Debie P, Declerck NB, van Willigen D, Huygen CM, De Sloovere B, Mateusiak L, Bridoux J, Puttemans J, Devoogdt N, van Leeuwen FWB, Hernot S. The Design and Preclinical Evaluation of a Single-Label Bimodal Nanobody Tracer for Image-Guided Surgery. Biomolecules. 2021 Feb 26;11(3):360. doi: 10.3390/biom11030360. PMID: 33652977; PMCID: PMC7996797.

https://dx.doi.org/10.3390%2Fbiom11030360

Hu Q,Wang K, Qiu L. 6-Aminocaproic acid as a linker to improve near-infrared fluorescence imaging and photothermal cancer therapy of PEGylated indocyanine green. Colloids Surf B Biointerfaces. 2021 Jan;197:111372. doi: 10.1016/j.colsurfb.2020.111372.

https://dx.doi.org/10.1016/j.colsurfb.2020.111372

Renard E, Dancer PA, Portal C, Denat F, Prignon A, Goncalves V. Design of Bimodal Ligands of Neurotensin Receptor 1 for Positron Emission Tomography Imaging and Fluorescence-Guided Surgery of Pancreatic Cancer. J Med Chem. 2020 Mar 12;63(5):2426-2433. doi: 10.1021/acs.jmedchem.9b01407. Epub 2020 Jan 2. PMID: 31855417.

https://dx.doi.org/10.1021/acs.jmedchem.9b01407