KAER LABS
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Kaer Imaging System
OPEN FLUORESCENCE IMAGING SYSTEM
The Kaer Imaging System is a stand alone Open Fluorescence In Vivo Imaging System. It is designed for the detection of near infrared fluorescent molecules in animals in real time. Its optical head can be hand held or fixed on a stand, depending on the setup of the study. The system can be used on both small and large animals and is adapted to intraoperative imaging.
The system is designed for preclinical research only and should not be used for clinical use
Imaging of subcutaneous tumors in a mouse model of pancreatic cancer with a bimodal PET-fluorescent tracer. Courtesy of Aurélie Prignon, Plateforme LIMP, UMS 28, Faculté de médecine Sorbonne Université, Paris, France, and Emma Renard, Institut de Chimie Moléculaire de l’Université de Bourgogne, UMR CNRS 6302, Université Bourgogne Franche-Comté, Dijon, France.
Open fluorescence imaging experiments with Dr. Aurélie Prignon at the LIMP imaging platform in Paris, developing new imaging agents for fluorescence guided surgery with the Kaer Imaging System
Specifications
KIS 700: excitation 640 nm - collection: > 665 nm (high pass)
KIS 800: excitation 785 nm - collection: > 800 nm (high pass)
Excitation type: laser
Field of view: 6.4 x 6.4 cm2
Image size (pixels): 1024 x 1024
Number of bits: 16
Image format: TIFF
Export format: AVI
Flexibility
Open design
Optical head - hand held or fixed on a stand
Dynamic imaging
Portable instrumentation
Can be controlled by a laptop
Sensitivity
Laser excitation
High power due to limited working distance
Dedicated filters to maximize sensitivity for a given wavelength (no filter wheel)
Near infrared fluorescence for better tissue propagation
Background recording and subtraction from fluorescence signal in real time
Pseudo colors and overlay in real time for easier image interpretation
Quantification
Linear detection for quantification of the signal
High dynamic range of the detector, for quantification of weak and strong signals
On the fly quantification of ROI: histograms and signal kinetics
Tif image format recording for post acquisition quantification
User friendliness
Very easy to install and use
Only one parameter to adjust to optimize image quality and sensitivity
No proprietary image format, post acquisition analysis is possible with any scientific image processing software
Bibliography
Here is the list of publications related to the KIS for Near Infrared fluorescence. The complete list of publications, including NIR-II fluorescence related articles, can be found here.
Holm-Weber T, Kristensen RE, Mohanakumar S, Hjortdal VE. Gravity and lymphodynamics. Physiol Rep. 2022 May. doi: 10.14814/phy2.15289
https://dx.doi.org/10.14814/phy2.15289
Privat M, Bellaye PS, Lescure R, Massot A, Baffroy O, Moreau M, Racoeur C, Marcion G, Denat F, Bettaieb A, Collin B, Bodio E, Paul C, Goze C. Development of an Easily Bioconjugatable Water-Soluble Single-Photon Emission-Computed Tomography/Optical Imaging Bimodal Imaging Probe Based on the aza-BODIPY Fluorophore. J Med Chem. 2021 Aug 2. doi: 10.1021/acs.jmedchem.1c00450.
https://doi.org/10.1021/acs.jmedchem.1c00450
Debie P, Declerck NB, van Willigen D, Huygen CM, De Sloovere B, Mateusiak L, Bridoux J, Puttemans J, Devoogdt N, van Leeuwen FWB, Hernot S. The Design and Preclinical Evaluation of a Single-Label Bimodal Nanobody Tracer for Image-Guided Surgery. Biomolecules. 2021 Feb 26;11(3):360. doi: 10.3390/biom11030360.
https://dx.doi.org/10.3390%2Fbiom11030360
Hu Q,Wang K, Qiu L. 6-Aminocaproic acid as a linker to improve near-infrared fluorescence imaging and photothermal cancer therapy of PEGylated indocyanine green. Colloids Surf B Biointerfaces. 2021 Jan;197:111372. doi: 10.1016/j.colsurfb.2020.111372.
https://dx.doi.org/10.1016/j.colsurfb.2020.111372
Renard E, Dancer PA, Portal C, Denat F, Prignon A, Goncalves V. Design of Bimodal Ligands of Neurotensin Receptor 1 for Positron Emission Tomography Imaging and Fluorescence-Guided Surgery of Pancreatic Cancer. J Med Chem. 2020 Mar 12;63(5):2426-2433. doi: 10.1021/acs.jmedchem.9b01407.
https://dx.doi.org/10.1021/acs.jmedchem.9b01407