top of page


NIR-II Kaer Imaging System


Fluorescence imaging, compared to other modalities (eg. bioluminescence, MRI or nuclear imaging) is limited by the depth of penetration of photons in tissue, even in the near infrared part of the spectrum. Imaging in the second biological second window, also called NIR-II, between 900 nm and 1700 nm, brings a significant improvement regarding the depth at which signals can be detected and the resolution of resulting images. Also, it becomes possible to consider imaging through thin bone structures, like mouse skulls.

With the same philosophy we had developed our first imaging system for Near Infrared (NIR) fluorescence, Kaer Labs now also proposes its stand alone Open Fluorescence In Vivo Imaging System for NIR-II. The system benefits from the same flexibility and user friendliness as the original KIS for NIR-I but will help designers of new fluorescent agents to push further the limits of In Vivo Fluorescence imaging.

The system is designed for preclinical research only and should not be used for clinical use


The Kaer Imaging System for NIR-II fluorescence

Read the article about NIR-II fluorescence imaging at the Small Animal Optical Imaging Laboratory (LIOPA, Paris)

Different names for the same technique

The second biological window corresponds to the spectrum comprised between 900 nm and 1 700 nm. It is also referred to as the "near infrared 2", or NIR-II, region. It requires the use of InGaAs cameras, which are called SWIR cameras by their manufacturers, SWIR standing for Short Wave Infrared.

Imaging in the second biological window, NIR-II imaging and SWIR imaing are therefore three different phrases that refer to the same technique.​

Vascularisation imaging in a mouse after injection of nanoparticles


Excitation: 808 nm and / or 980 nm

Collection: 1000 nm, 1100 nm, 1200 nm, 1300 nm, 1400 nm, 1500nm (high pass filters)

Excitation type: laser

Field of view: 6.4 x 6.4 cm2

Image size (pixels): 512 x 512

Number of bits: 16

Image format: TIFF

Export format: AVI



Open design

Optical head - hand held or fixed on a stand

Dynamic imaging

Portable instrumentation

Can be controlled by a laptop


Laser excitation

High power due to limited working distance

Dedicated filters to maximize sensitivity for a given wavelength (no filter wheel)

Near infrared fluorescence for better tissue propagation

Background recording and subtraction from fluorescence signal in real time

Pseudo colors and overlay in real time for easier image interpretation



Linear detection for quantification of the signal

High dynamic range of the detector, for quantification of weak and strong signals

On the fly quantification of ROI: histograms and signal kinetics

Tif image format recording for post acquisition quantification


User friendliness

Very easy to install and use

Only one parameter to adjust to optimize image quality and sensitivity

No proprietary image format, post acquisition analysis is possible with any scientific image processing software


Here is the list of publications related to the KIS for Near Infrared fluorescence. The complete list of publications, including Near Infrared (NIR-I) fluorescence related articles, can be found here.

Yao K, Mu Q, Zhang Y, Cheng Q, Cheng X, Liu X, Luo C, Li C, Cai S, Luo Z, Zhu X, Tang, L. Hesperetin Nanoparticle Targeting Neutrophils for Enhanced TBI Therapy. Advanced Functional Materials, 2205787. 2022. doi: 10.1002/adfm.202205787

Rodríguez-Luna MR, Okamoto N, Al-Taher M, Keller DS, Cinelli L, Hoskere Ashoka A, Klymchenko AS, Marescaux J, Diana M. In Vivo Imaging Evaluation of Fluorescence Intensity at Tail Emission of Near-Infrared-I (NIR-I) Fluorophores in a Porcine Model. Life (Basel). 2022 Jul 27;12(8):1123. doi: 10.3390/life12081123

Yu Z, He Y, Schomann T, Wu K, Hao Y, Suidgeest E, Zhang H, Eich C, Cruz LJ. Achieving Effective Multimodal Imaging with Rare-Earth Ion-Doped CaF2 Nanoparticles. Pharmaceutics. 2022 Apr 11;14(4):840. doi: 10.3390/pharmaceutics14040840

Liu Q, Zhong Y, Su Y, Zhao L, Peng J. Real-Time Imaging of Hepatic Inflammation Using Hydrogen Sulfide-Activatable Second Near-Infrared Luminescent Nanoprobes. Nano Lett. 2021 Jun 9;21(11):4606-4614. doi: 10.1021/acs.nanolett.1c00548. Epub 2021 May 20. PMID: 34014668.

bottom of page