KAER LABS
3D ORGAN IMAGING
Biomaterials
BIOPRINTED CUBE
Aim: Characterize internal structure of bioprinted materials to study material composition over time as a result of cellular colonization and extracellular matrix (ECM) production
Approach: Cryosectionning of a bioprinted cube containing mCherry endothelial cells.
Result: Validation of cryopreservation protocols of the hydrogels and observation of the fluorescent cells. Visualisation of a neo-vascular organisation of the cells within the gel after 21 days in culture.
A
B
Figure: A.) Autofluorescence signal of a bioprinted cube at 470nm, 2 x 2 cm. B.1) Cryosectionning and imaging at 535nm and B.2 image segmentation to visualize the fluorescence signal of mCherry endothelial cells. C) Z view of a reconstructed cube imaged at 470 and 535 nm (overlay). Neovascularisation can be observed at the edges of the cube.
Courtesy of Lucie Essayan, Emma Petiot, Elliott Cowles, Université de Lyon, ICBMS, 3D Fab, Villeurbanne, France
Kinsight as a service
In collaboration with the microscopy and histology platform MicroPICell from Nantes University / SFR François Bonamy, Kaer Labs can propose the technique as a service. It is as simple as shipping us your organs embedded in paraffin or frozen in OCT. Contact us for more information on prices and processes.